Recombinant proteins expressed in bacteria often form inclusion bodies, especially when they are expressed at high levels. Creative BioMart retrieves expressed GST fusion protein in soluble form after lysis and extraction procedures.

Advantages:
. Specifically for solubilizing and renaturing GST inclusion bodies.
. Time-saving: without lengthy dialysis or dilution step.
. No pH variation and Redox Pair involved.

Refolding of the solubilized proteins is initiated by the removal of the denaturant. The